Cell Sorting Flow Cytometry

Michael timm s pearl on cell sorting using flow cytometry explains the origins of cell sorting technology the two main types and their differences as well as best practices and troubleshooting sorting assays.

Cell sorting flow cytometry.

Forward scatter fsc forward light scatter refers to the refracted light past the cell into a detector behind the laser. Hello my name is michael timm. For example if lymphocytes were concentrated to 20 million per ml the flow rate at the instrument could be run as high as 20 000 cells per second. Cells larger than lymphocytes require a larger nozzle to be placed on the instrument and the flow rate is lower.

Download slides pdf transcript. Using flow cytometry requires a flow cytometric cell sorter like the bd facsaria. Download transcript pdf slide 1. With flow cytometry cell sorting multiple detectors are being utilized to analyze different cell characteristics.

One is by using flow cytometry and the other is by magnetically labeling the cells to differentiate and separate the cell populations. There are two methods for performing cell sorting. Researchers partnering with nyu langone s cytometry and cell sorting laboratory have access to the most powerful and adaptable flow cytometry and cell sorting technologies and instruments available. Cell sorting is the separation and isolation of various cell populations.

12 14 31 in flow cytometers with sorting capabilities the instrument detects cells using parameters including cell size morphology and protein expression and then droplet technology.