Total flask area cm2 cell yield ml flask volume ml 25 cm2 25 2 5 x 106 5 7 5 70 rectangular 75 cm2 75 7 5 x 106 15 22 5 265 u shaped 150 cm2 150 1 5 x 107 30 45 377 u shaped 175 cm2 175 1 75 x 107 35 52 5 513 u shaped 225 cm2 225 2 25 x 107 45 67 5 1 006 traditional. This process improves cell attachment by incorporating significantly more oxygen into the cell culture surface rendering it more hydrophilic. Surface areas of cell culture vessels the following are the surface areas of various tissue culture dishes or plates and their relative size to a 35 mm dish.
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